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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: J Neurosurg. 2015 Jan;122(1):69–77. doi: 10.3171/2014.9.JNS132373

Figure 3.

Figure 3

Cadherin-19 is specifically expressed in minimally infiltrative GSCs. (A): Heat map of qRT-PCR validation of all candidate GSC cell surface markers. Additional GSC lines, 33 & 44 GSCs, were evaluated compared to hNSCs, normal human astrocytes (NHA), U87 glioma cells, and patient matched, serum-cultured GBM tumor cells 22T and 33T. Data is representative of two experimental repetitions normalized to hNSC, and reported as a fold change value with accompanying color key. (B): Western blot of cadherin-19 (CDH19) specifically expressed in 12.1 and 22 GSCs. Ms-pAb-anti-CDH19 (Abnova, H00028513-B01P) at 1:250, identified at ∼114k kDa. Rb-pAb-anti-β-actin (Abcam, ab8227) at 1:2000 was used as a loading control.