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. Author manuscript; available in PMC: 2015 Feb 23.
Published in final edited form as: Anal Chem. 2008 Dec 15;80(24):9830–9834. doi: 10.1021/ac8021899

Figure 2.

Figure 2

Top: Tracking microscope collection efficiency function (CEF) near Z=0 measured through 4 fibers. Transmitted emission: fibers/detectors D0 and D1. Reflected emission: fibers/detectors D2 and D3. Data collected from a quantum dot immobilized in polydimethylsiloxane. Peak count rates are normalized to 1. For a single quantum dot at the origin, the count rate is ~ 64 kHz spread amongst all four detectors. Bottom: Error signals (Solid Squares) from Equation 1 for the three Cartesian axes calculated from line scans through the experimental CEF. Black solid lines are linear fits to the linear portion of the error signals. The linear slopes for X,Y and Z are −3.7,−3.0.−0.90 respectively. Error signals interpolated from the scanned CEF data.