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. 2015 Feb 23;10(2):e0115828. doi: 10.1371/journal.pone.0115828

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Fig 6 — Wild type C57Bl/6 or C57Bl/6 ager-/- mice were challenged intranasally with PBS, or adeno-null or adeno-murine S100A9, after 8 days, mice were sacrificed and BAL fluids or lung tissue were collected for analysis. (A) Cells were obtained from the BAL fluids using cytospins, stained with diff-quik and the total cell counts, neutrophil counts and macrophage counts were recorded. (B) mIFNγ and mIL-6 proteins levels found in BAL fluids of wild type and ager-/- mice. (C) Western blot analysis of murine S100A9 levels in BAL fluids. (D) H&E staining of paraffin fixed lung tissue (left panel), and pathology scores (right panel). Non-parametric Mann-Whitney test was used to determine statistical difference between two groups.