Fig 3. PCR analyses of semen DNA from G₀ males with an integrated hLY fragment.

To estimate approximate copy numbers, control PCR reactions were performed in parallel, using 500 ng aliquots of chicken genomic DNA containing appropriate quantities of plasmid DNA to simulate a single-copy gene (1); a 10-fold dilution of a single-copy gene (0.1); a 100-fold dilution of a single-copy gene (0.01); and a 1000-fold dilution of a single-copy gene (0.001). 20 ng of genomic DNA were used for the amplification of the chicken housekeeping gene GAPDH. WT, wild-type White Leghorns.