Fig 4. The identification of transgenic chicken.

(A, B) Southern blot analyses of genomic DNA from individual G1 birds. Genomic DNA samples (10 μg) were digested with PvuII(A) or NdeI (B) to generate either a fragment (2.3 kb) spanning the vector or a junction fragment between viral and chicken genomic sequences respectively, then hybridised with a probe for hLY. (C) PCR analysis of genomic DNA from blood of G₂ offspring sired by A011. A011, A037 and D514, G₁ offspring of G₀ birds injected with pWPXL-EREOV-hLY. WT, wild-type White Leghorns. C1, plasmid control with a copy number of 1. C5, plasmid control with a copy number of 5. PC, positive control.