Table 1. Q-PCR analysis of semen DNA from hatched male chickens and the efficiency of germline transmission.

Viral titre (TU/ml)	G0 Bird no.	Semen copy number	G1 trasgenic offspring/total	Sex of positive G1
10 9	E00127	0.025	2/88(2.27%)	1♀1♂
10 10	E00135	0.090	0/84	—
10 10	E00137	0.005	1/311(0.32%)	1♂
10 10	E00149	0.028	1/60(1.67%)	1♂
10 10	E00174	0.098	1/169(0.59%)	1♀
10 11	C00650	0.083	0/118	—
Total	—	—	5/830(0.60%)	2♀3♂