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. 2015 Feb 23;10(2):e0118311. doi: 10.1371/journal.pone.0118311

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Fig 5 — Spleens were harvested from E8i-Cre⁺ SNAP-23^fl/+ control mice or E8i-Cre⁺ SNAP-23^fl/- mice (line E3) and single cell suspensions were stained with fluorochrome-conjugated CD3-, CD4-, and CD8-mAb and analyzed by flow cytometry. (A) A representative flow cytometry profile of CD3-gated spleen cells reveals a selective reduction in the percentage of CD8⁺ T cells in the spleen. (B) Quantitative analysis of E8i-Cre⁺ SNAP-23^fl/+ or E8i-Cre⁺ SNAP-23^fl/- littermate mice indicating total spleen cell numbers, B cell numbers (determined by gating on B220⁺ spleen cells) as well as the numbers of CD4 T cells and CD8 T cells present in each spleen (calculated based on the percentage of each cell type present as determined by flow cytometry). The data shown are mean +/- SD of three independent experiments (*p<0.05; **, p<0.01).