Table 5. Primers used in PCR reactions.
Target gene | Primer | Primer position | Amplicon size (bp) | Nucleotide sequence (5’ to 3’) | Reference(s) |
---|---|---|---|---|---|
MCP | OL-T1 | 97387–97404 | 531 | GACTTGGCCACTTATGAC | [8, 28] |
OL-T2R | 97917–97899 | GTCTCTGGAGAAGAAGAAT | |||
MCP-BF | 97813–97830 | 548 | ACCAGCGATCTCATCAAC | [14] | |
MCP-BR | 98360–98341 | AGCGCTGGCTCCAGGACCGT | |||
MCP-5 | 98244–98263 | 585 | CGCAGTCAAGGCCTTGATGT | [12] | |
MCP-6R | 98828–98807 | AAAGACCCGTTTTGCAGCAAAC | |||
DNApol | DNApol-F | 67188–67208 | 560 | GTGTAYCAGTGGTTTTGCGAC | [13] |
DNApol-R | 67747–67728 | TCGTCTCCGGGYCTGTCTTT | |||
RNR-α | RNR-AF | 43729–43748 | 806 | CTGCCCATCTCKTGCTTTCT | [14] |
RNR-AR | 44534–44513 | CTGGCCCASCCCATKGCGCCCA | |||
RNR-β | RNR-BF | 78029–78012 | 646 | AGGTGTRCCRGGGYCGTA | [14] |
RNR-BR | 77384–77403 | GACGCTCCAYTCGACCACTT | |||
vIF-2α | vIF-2αF | 32950–32969 | 247 or 1050 | AAATGCAATGACTGTAAATG | [35], Chinchar, pers.comm. |
vIF-2αR | 33181–33208 | GGCCAAGCTTTTACACAAAGGGGCACA | |||
Microsatellite region | CMTVre_F | 80807–80824 | variable | TCTTTACTCCATCGCACA | [18] |
CMTVre_R | 80913–80930 | ACGCACTGAAAAGGTGCA | |||
Genomic arrangement | GenAr_1 | 14956–14980 | see Table 4 | GTTTGCAGAGCGTCAGCTCGTGGAC | |
GenAr_2 | 102673–102699 | CACGAAAACTGGCAGCTGAGGGACGCC | |||
GenAr_3 | 15849–15824 | GCATGCGCAAGTCTGCCGAGGCGGTC | |||
GenAr_4 | 103579–103551 | GTGAAAGGATTGCGATAAACTGAGACCAC | |||
GenAr_5 | 29321–29296 | GACACAATCCAGCTCGTCTGTGAGAC | |||
GenAr_6 | 28861–28889 | GACTGTAGACGGCTGGCCAGGGTACGCCG |