. 2015 Feb 23;10(2):e0118633. doi: 10.1371/journal.pone.0118633

Table 5. Primers used in PCR reactions.

Target gene	Primer	Primer position	Amplicon size (bp)	Nucleotide sequence (5’ to 3’)	Reference(s)
MCP	OL-T1	97387–97404	531	GACTTGGCCACTTATGAC	[8, 28]
	OL-T2R	97917–97899	531	GTCTCTGGAGAAGAAGAAT	[8, 28]
	MCP-BF	97813–97830	548	ACCAGCGATCTCATCAAC	[14]
	MCP-BR	98360–98341	548	AGCGCTGGCTCCAGGACCGT	[14]
	MCP-5	98244–98263	585	CGCAGTCAAGGCCTTGATGT	[12]
	MCP-6R	98828–98807	585	AAAGACCCGTTTTGCAGCAAAC	[12]
DNApol	DNApol-F	67188–67208	560	GTGTAYCAGTGGTTTTGCGAC	[13]
DNApol	DNApol-R	67747–67728	560	TCGTCTCCGGGYCTGTCTTT	[13]
RNR-α	RNR-AF	43729–43748	806	CTGCCCATCTCKTGCTTTCT	[14]
RNR-α	RNR-AR	44534–44513	806	CTGGCCCASCCCATKGCGCCCA	[14]
RNR-β	RNR-BF	78029–78012	646	AGGTGTRCCRGGGYCGTA	[14]
RNR-β	RNR-BR	77384–77403	646	GACGCTCCAYTCGACCACTT	[14]
vIF-2α	vIF-2αF	32950–32969	247 or 1050	AAATGCAATGACTGTAAATG	[35], Chinchar, pers.comm.
vIF-2α	vIF-2αR	33181–33208	247 or 1050	GGCCAAGCTTTTACACAAAGGGGCACA	[35], Chinchar, pers.comm.
Microsatellite region	CMTVre_F	80807–80824	variable	TCTTTACTCCATCGCACA	[18]
Microsatellite region	CMTVre_R	80913–80930	variable	ACGCACTGAAAAGGTGCA
Genomic arrangement	GenAr_1	14956–14980	see Table 4	GTTTGCAGAGCGTCAGCTCGTGGAC
	GenAr_2	102673–102699		CACGAAAACTGGCAGCTGAGGGACGCC
	GenAr_3	15849–15824		GCATGCGCAAGTCTGCCGAGGCGGTC
	GenAr_4	103579–103551		GTGAAAGGATTGCGATAAACTGAGACCAC
	GenAr_5	29321–29296		GACACAATCCAGCTCGTCTGTGAGAC
	GenAr_6	28861–28889		GACTGTAGACGGCTGGCCAGGGTACGCCG

The primer positions presented are relative to the FV3 genome (AY548484); primers of the genomic arrangement sites are relative to CH8/96 (gb KP266741).

Y = C/T, K = G/T, S = C/G, R = A/G