Skip to main content
. 2015 Feb 23;10(2):e0117841. doi: 10.1371/journal.pone.0117841

Fig 3. Expression analyses of brown and beige markers in adipose tissue samples of adults with low compared to high UCP1 mRNA expression.

Fig 3

We compared the upper 10% with the lowest 10% of UCP1 mRNA expression in paired subcutaneous and visceral AT samples of adults (n = 48) (A). Subcutaneous and visceral AT samples of adults with a high UCP1 expression showed significantly increased mRNA levels of PRDM16 (B) and PAT2 (C) in both subcutaneous and visceral depots. The classical brown specific marker ZIC1 was not altered between subcutaneous/visceral samples with a low or high UCP1 expression (D). Furthermore, samples with a high UCP1 expression appear to have slightly higher TMEM26 expression in subcutaneous and significantly increased mRNA levels in visceral AT samples (E). In contrast, TBX1 levels were significant increased in subcutaneous depots and slightly in visceral depots (F). Expression levels were normalized with the reference gene RPL27. Statistical significance was assessed by Student t-test and was marked with *P<0.05; **P< 0.01; ***P<0.001.