Table 1. Assembly of total RNA-seq and polyribosomal RNA-seq and AS estimates.

	No. of Assembled transcripts		No. of transcripts after filtering		PASA Assembly
	De novo	Genome-guided	De novo	Genome-guided
Total RNA	42,962	38,340	23,697	37,785	34,231
Poly RNA	35,545	29,461	35,259	29,298	26,959
		Alternative splicing estimates
		Total RNA sample		Polyribosomal RNA sample
Genes detected in samples		12,876		12,515
Intron-containing genes		11,967		11,571
Alternatively Spliced genes		2,090		1,396
% of genes with AS		17.5%		12.1%