Fig 4. Effect of dasatinib, tamoxifen and fulvestrant on growth of parental and resistant T47D cells.

(A, B) Parental and resistant cell lines were grown for 5 days with vehicle (0.1% DMSO), 1 μM dasatinib (Das), 100 nM fulvestrant (Fulv) or 1 μM tamoxifen (Tam) alone or in the indicated combinations. (C) Tamoxifen resistant cell lines were treated for five days with 100 nM fulvestrant and dasatinib at the indicated concentrations. Cell number was measured by a crystal violet colorimetric assay and expressed as percentage of untreated control. A representative experiment out of three with mean ± SD is shown. * P<0.05 for treated vs untreated cells; # P<0.05 for cells treated with dasatinib vs cells treated with a combination of dasatinib and tamoxifen or fulvestrant.