Fig 5. Effect of dasatinib, tamoxifen and fulvestrant alone or in the indicated combinations on long-term propagation of parental and tamoxifen resistant cell lines.
T47D/S2 (A, B), TR-1 (C, D) and TR-2 (E, F) cells were seeded with 4x105 cells in T25-flasks and treated for one week with 1 μM dasatinib (Das), 1 μM tamoxifen (Tam) or 100 nM fulvestrant (Fulv) alone or in combination as indicated in the figure. Control cells were propagated in medium containing DMSO and/or ethanol (vehicle) corresponding to the same amount as treated cultures. Growth rate was determined as the ratio between cell number after one week treatment and number of seeded cells. After cell number determination, 4x105 cells were reseeded in T25-flasks and allowed to grow for additional two weeks with weekly split and determination of cell number. In cases with fewer than 4x105 cells, the total number of cells were added and used for the calculations. w/o; without.