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. 2015 Feb 23;9(2):e0003454. doi: 10.1371/journal.pntd.0003454

Fig 3. Expression and purification analysis of rSjPGM (A) or rSjRAD23 (B) protein using SDS-PAGE (12%).

Fig 3

Lane M: Molecular mass markers. Lane 1: Total extract from pET28a(+) after induction with 1 mM IPTG. Lane 2: Total extract from rSjPGM or rSjRAD23 after induction with 1 mM IPTG. Lane 3: rSjPGM or rSjRAD23 purified through Ni2+-charged column chromatography.