Table 2. MALDI-TOF-TOF MS identification of some TG proteins which may be potential diagnostic antigens for schistosomiasis japonicum.
Spot ID | Accession no. | Description | Theoretical Mr/pI | Protein score | Protein score C.I.% | Sequence coverage (%) a | No. of peptides matched |
---|---|---|---|---|---|---|---|
1–3 | gi|56752663 | Putative heat shock protein | 52487/5.91 | 395 | 100 | 29.98 | 12 |
4 | gi|226470142 | UV excision repair protein RAD23 homolog B (RAD23) | 37914/4.46 | 82 | 99.969 | 34.00 | 7 |
5 | gi|29841387 | similar to GenBank Accession Number J04017 heat shock protein 86 in Schistosoma mansoni | 31154/6.77 | 92 | 99.997 | 27.27 | 5 |
6 | gi|226489240 | Charged multivesicular body protein 5 | 24997/4.63 | 475 | 100 | 25.89 | 6 |
7–9 | gi|226489007 | similar ot Metallo-beta-lactamase domain-containing protein 1 | 26537/4.84 | 324 | 100 | 43.64 | 8 |
10 | gi|226472666 | phosphoglycerate mutase (PGM) | 28474/6.32 | 529 | 100 | 52.80 | 11 |
a The percentage coverage was defined as the ratio (%) of the protein sequence covered by the matched peptides.