Fig 1. miR-214 expression was frequently attenuated in bladder cancer tissues and associated with poor progonosis.

(A) Expression level of miR-214 was determined by means of qRT-PCR and normalized against U6 RNA, an endogenous control, in 106 pairs of human bladder cancer tissues (BC) and matched adjacent normal tissues (NT). ***P < 0.001, Wilcoxon test, n = 106. (B) The attenuated expression of miR-214 was found in 74% (78 of 106) of bladder cancer tissues compared with adjacent normal specimens. (C) Relative expression of miR-214 was presented as log₂ fold change (BC/NT) and the log₂ fold change was defined as follows: <1, underexpression; >1, overexpression; the remaining was defined as unchanged. (D) Kaplan-Meier RFS and OS curves based on the miR-214 expression levels of patients with bladder cancer. The cut-off point was the median miR-214 expression level in the bladder cancer tissue samples. (E) Kaplan-Meier RFS and OS curves based on the miR-214 expression levels of patients with MIBC. The cut-off point was the median miR-214 expression level in the MIBC tissue samples.