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. 2015 Feb 23;10(2):e0118458. doi: 10.1371/journal.pone.0118458

Table 1. Primer sequences for RT-PCR.

Gene name NCBI Accession number Product size (bp) Primer Sequence (5’-3’) Sense/antisense
IL-6 NM_173923.2 153 TGCTGGTCTTCTGGAGTATC
GTGGCTGGAGTGGTTATTG
IL-1β  NM_174093.1 264 CGTCTTCCTGGGACATTTTCG
GTCTGAGGATGGGCTCTGGG
MD-2 AB072456.1 276 ACCGTTTGGTACGACTACTGTGAT
CCTGAAGGAGAATTGTATTGTTGTG
NF-kB XM_005226181.1 249 AAGAGAAGATGGGGAAAGGCTG
CGTCGGCAAATGAGAAGTAGTG
IL-12 AJ308426.2 238 ATTTATGTTCAGCATGGTCACTCC
CAGTGTACAGGTGGGTGTCTCG
LBP NM_001038674.2 108 TCAAGGGCATCACCAT
GCACCTCCACATCACG
CD14 NM_174008.1 109 ACCACCCTCAGTCTCCGTAA
GTGCTTGGGCAATGTTCAG
MyD88 NM_001014382.2 177 GCAGCATAACTCGGATAAA
CAGACACGCACAACTTCA
TLR4 NW_003103900.1 166 TCCCACATCCTCGGTTCCC 
TCCATCCCAAGCCATCCCT 
TNF-a NM_173966 175 ACCCAGCCAACAGAAGC
CCAGACGGGAGACAGGA
18S rRNA NM_174841.2 116 TCCAGCCTTCCTTCCTGGGCAT
GGACAGCACCGTGTTGGCGTAGA
IL-10 NM_174088 110 GCCACAGGCTGAGAACC
TTTCACTGCCTCCTCCAGAT