Table 1. Primer sequences for RT-PCR.
Gene name | NCBI Accession number | Product size (bp) | Primer Sequence (5’-3’) Sense/antisense |
---|---|---|---|
IL-6 | NM_173923.2 | 153 | TGCTGGTCTTCTGGAGTATC |
GTGGCTGGAGTGGTTATTG | |||
IL-1β | NM_174093.1 | 264 | CGTCTTCCTGGGACATTTTCG |
GTCTGAGGATGGGCTCTGGG | |||
MD-2 | AB072456.1 | 276 | ACCGTTTGGTACGACTACTGTGAT |
CCTGAAGGAGAATTGTATTGTTGTG | |||
NF-kB | XM_005226181.1 | 249 | AAGAGAAGATGGGGAAAGGCTG |
CGTCGGCAAATGAGAAGTAGTG | |||
IL-12 | AJ308426.2 | 238 | ATTTATGTTCAGCATGGTCACTCC |
CAGTGTACAGGTGGGTGTCTCG | |||
LBP | NM_001038674.2 | 108 | TCAAGGGCATCACCAT |
GCACCTCCACATCACG | |||
CD14 | NM_174008.1 | 109 | ACCACCCTCAGTCTCCGTAA |
GTGCTTGGGCAATGTTCAG | |||
MyD88 | NM_001014382.2 | 177 | GCAGCATAACTCGGATAAA |
CAGACACGCACAACTTCA | |||
TLR4 | NW_003103900.1 | 166 | TCCCACATCCTCGGTTCCC |
TCCATCCCAAGCCATCCCT | |||
TNF-a | NM_173966 | 175 | ACCCAGCCAACAGAAGC |
CCAGACGGGAGACAGGA | |||
18S rRNA | NM_174841.2 | 116 | TCCAGCCTTCCTTCCTGGGCAT |
GGACAGCACCGTGTTGGCGTAGA | |||
IL-10 | NM_174088 | 110 | GCCACAGGCTGAGAACC |
TTTCACTGCCTCCTCCAGAT |