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. 2015 Feb 23;10(2):e0117662. doi: 10.1371/journal.pone.0117662

Fig 4. BN-PAGE and immunoblot analyses.

Fig 4

(A) Separation and identification of the pigment-protein complexes in first and second true leaves of 2-week old WT and atl31–1/atl6–1 plants grown under ML and LL conditions. The BN-PAGE gels were destained with 20% MeOH and 7% acetic acid to clarify the bands of the pigment-protein complexes. The PSII dimer (PSII-D), PSI-LHCI supercomplex, RubisCO complex, CP29-CP24-LHCII trimer (LHCII assembly), LHCII trimer (LHCII-T) and LHCII monomer (LHCII-M) were visualized in the BN-gel. Proteins were identified as described [25,32,33]. The positions of the molecular markers are indicated on the right. (B) Immunoblot analyses and CBB staining of the plastid proteins in first and second true leaves of 2-week-old WT and atl31–1/atl6–1 plants grown under ML conditions. Total leaf extracts were loaded onto 14% SDS—PAGE gels containing 4M urea. Immunoblot analyses were performed using antibodies to PsbC, PsbD, Lhcb1 and PsaA/B, whereas RbcL was visualized by CBB staining. A dilution series of the WT samples is indicated in percentage.