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. 2015 Feb 23;10(2):e0118205. doi: 10.1371/journal.pone.0118205

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© 2015 Karah et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — A) The CRISPR-cas subtype I-Fb locus in A. baumannii strain AYE (GenBank accession number: CU459141, located at position 1,057,691 to 1,069,768). The locus consisted of two CRISPR–associated genes (cas1 and cas3/cas2), four Cas system-associated genes (csy1, csy2, csy3, and csy4), and an array of spacers. The map was created using Artemis (http://www.sanger.ac.uk/resources/software/artemis/). B) Nucleotide sequence of the array of spacers in A. baumannii strain AYE. The array included 59 spacers surrounded by 60 direct repeats (marked in green). Some repeats, mainly at the trailer end of the array, included degenerated nucleotides (marked in yellow). C) Comparative analysis of the genetic surroundings. The comparison was performed between the locus-positive strain AYE which belonged to sequence type (ST1) and the locus-negative strains TYTH-1, AB4857, and OIFC099 which belonged to ST2, ST3, and ST32, respectively. Homologous sequences shared by all the isolates were indicated by gray zones.