Table 3. qPCR primer sensitivities and reaction efficiencies.
Species DNA | Primer | Efficiency % | r2 |
---|---|---|---|
C. albicans | Uni1 | 101.0 | 0.999 |
C. albicans | Calb | 103.1 | 0.999 |
C. glabrata | Cgla | 112.1 | 0.998 |
C. parapsilosis | Cpar | 102.7 | 0.992 |
C. tropicalis | Ctro | 111.1 | 0.994 |
C. krusei | Ckru | 112.1 | 0.998 |
Efficiencies and r2 values determined using the log-linear regions of standard curves generated using 10-fold serial dilutions of Candida species genomic DNA.