Table 4. Comparison of data from sequencing and qPCR for eleven infant fecal samples.
| Sequencing results* | qPCR results by primer* | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Sample | Mean reads | StDev Reads | Mean Shannon | StDev Shannon | C. albicans | C. parapsilosis | C. tropicalis | C. krusei | Uni | Calb | Cpar | Ckru |
| 17 | 2259 | 2133 | 2.25 | 0.16 | +++ | +++ | −−− | +++ | +++ | +++ | +++ | + |
| 21 | 282972 | 351107 | 0.79 | 0.22 | +++ | +++ | ++− | +++ | +++ | +++ | +−− | − |
| 24 | 1764 | 1917 | 1.57 | 0.74 | +++ | ++− | +−− | ++− | +++ | +−− | −− | −− |
| 25 | 2087 | 3443 | 1.76 | 0.82 | +++ | ++− | −−− | −−− | +++ | ++− | −− | −− |
| 27 | 338 | 452 | 1.72 | 0.20 | +++ | ++− | −−− | +−− | +++ | +++ | −− | − |
| 28 | 2248 | 2509 | 2.19 | 0.18 | +++ | +++ | +−− | −−− | +++ | +++ | −− | − |
| 30 | 1911 | 1728 | 2.03 | 0.11 | +++ | +++ | ++− | −−− | +++ | ++− | −− | − |
| 31 | 2406 | 1616 | 1.80 | 0.26 | +++ | ++− | +−− | −−− | +++ | +−− | −− | − |
| 32 | 3016 | 3651 | 1.81 | 0.40 | +++ | +++ | −−− | ++− | ++− | +++ | −− | − |
| 34 | 3009 | 2204 | 1.78 | 0.38 | +++ | ++− | −−− | +−− | +++ | ++− | −− | − |
| 35 | 1078 | 988 | 1.55 | 0.55 | +++ | ++− | −−− | +−− | +++ | ++− | −− | − |
* +, positive qPCR signal (above the LoD) in one replicate sample;-, no qPCR signal (below the LoD). As shown, most determinations were performed in triplicate; some determinations were done in duplicate or singly due to lack of template material.