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. 2015 Feb 23;10(2):e0118806. doi: 10.1371/journal.pone.0118806

Fig 3. Neutralization of MYXV virions.

Fig 3

MYXV virions were neutralized by the sialic acid-specific lectin MAA (Maackia amurensis) and by the alternative pathway of complement. A. MYXV WT, M138L Del, M138L STOP and M138L Rev mature virions (MVs) were incubated with dilutions of MAA lectin. After incubation (2h, 37°C) the viruses were plaque assayed for infectivity on RK13 cells. Data are expressed as plaque number relative to untreated equivalent virus samples. Data are the average +/- SEMs for 2 independent experiments and were analyzed by 2way ANOVA and Bonferroni post-tests. B-C. MYXV WT, M138L Del, M138L STOP and M138L Rev mature virions (MVs) (B) or freshly-prepared extracellular virions (EVs) (C) were incubated with serum from naïve rabbits, either untreated (continuous lines) or heat-inactivated (dashed lines). After incubation (2h, 37°C) the viruses were plaque assayed for infectivity on RK13 cells. Data are the average +/- SEMs for 3 independent experiments and were analyzed by 2way ANOVA and Bonferroni post-tests. No significant difference was observed between the different viral strains.