Table 3. Primer Information.
| Name | Target species | Type of assay | Forward | Reverse | Probe (TaqMan markers only) | Length (bp) |
|---|---|---|---|---|---|---|
| SC-1 | SC | cPCR | GGACCCAGTACTATTAACTGCTCTA | TCCTAGGGCAAGGAGGGTA | 171 | |
| SC-5 | SC | cPCR | TCCGATTACCGCCACAATTATAGCCTTAG | GATAGGGTTAGTGGAAGAGAGGAC | 161 | |
| SC-7 | SC | cPCR | ACTGAATAAACACACACATGTTCGAT | ATCATCACCCGATTAGTAAAAATG | 275 | |
| SC-TM4 | SC | qPCR | CCACTAACATCACCACGCAA | AGCCTTTTCCAGAGGCTTGG | TAACCCAGCTGCCAATACAA | 168 |
| SC-TM5 | SC | qPCR | CCACAACTTACCCTCCTTGCC | AAGGGTATTAATTTTTGTGGTGGA | TCATGACATCCGCAGCATTCCTC | 98 |
| BH-6 | BHC | cPCR | CAATACCCTAGCAATTATTCCCTTA | TGTAATTCCAAGGGCGGTTAG | 375 | |
| BH-8 | BHC | cPCR | gatgtaaactatggctggcttatt | tgtagaaagaggaggtgtaggaa | 388 | |
| BH-TM1 | BHC | qPCR | TAGACCTTCTAACAGGACTAATTC | AATCCACCTCATCCTCCAAC | CCGCCCTTGGAATTACATCCACA | 144 |
| BH-TM2 | BHC | qPCR | CCTTCGTCAAACAGACCTTAAATCC | CCCTCATGGGGTTTGGATTAGA | CCACATAGGACTTGTAGCGGGTGGA | 96 |
| BH-TM4 | BHC | qPCR | CCACTAACATCGCCACGTAG | AACCTTTTCCAGAAGCTTGG | TAGCCCAGCCGCCAACACAA | 168 |
| AC-6 | Both | cPCR | GTTCCTAATCAGCACCTTAGTACTCT | AATTCGAAGGGATGGCAAG | 156 | |
| AC-TM1 | Both | qPCR | GGCCGGAACAGGATGAACAGTT | TAATAGTTGTGGTGATGAAGTTAATTG | CACGCAGGAGCATCCGTAGACCT | 145 |
| AC-TM2 | Both | qPCR | CAATTAACTTCATCACCACAACTATTA | TCCAGCAGCTAAAACTGGTAAGG | AAACACCTCTCTTTGTTTGAGCTGTGC | 133 |
| AC-TM3 | Both | qPCR | TTCATCGGCGTAAATCTTACAT | AGGGAAATAAGAGATCCGATAGA | ACCCAGATGCCTACGCCCTG | 133 |
Primer sequences of markers used for field and sensitivity testing. For targeted species, BHC = bighead carp, SC = silver carp, Both = primers that could potentially be used for non-specific detection of both BHC and SC.