Table 5. Sensitivity Trials.
| Marker | Type of assay | Dilution: | ||||||
|---|---|---|---|---|---|---|---|---|
| 10-1 | 10-2 | 10-3 | 10-4 | 10-5 | *10-6 | *10-7 | ||
| Silver Carp: | ||||||||
| QAPP-SC | cPCR | 1.00 | 1.00 | 1.00 | 1.00 | 0 | 0 | 0 |
| SC-1 | cPCR | 1.00 | 1.00 | 1.00 | 0.75 | 0 | 0 | 0 |
| SC-5 | cPCR | 1.00 | 1.00 | 1.00 | 1.00 | 0 | 0.50 | 0 |
| SC-7 | cPCR | 1.00 | 1.00 | 1.00 | 1.00 | 0 | 0 | 0 |
| SC-TM4 | qPCR | 1.00 | 1.00 | 1.00 | 1.00 | 0.25 | 0.25 | 0 |
| SC-TM5 | qPCR | 1.00 | 1.00 | 1.00 | 0.75 | 0.50 | 0.25 | 0.25 |
| Estimated Target-Sequence Copy Number: | 6051 | 508 | 46 | 3.4 | 1.6 | U | U | |
| Bighead Carp: | ||||||||
| QAPP-BH | cPCR | 1.00 | 1.00 | 1.00 | 1.00 | 0.25 | 0.25 | 0 |
| BH-6 | cPCR | 1.00 | 1.00 | 1.00 | 0.25 | 0 | 0 | 0 |
| BH-8 | cPCR | 1.00 | 1.00 | 1.00 | 0.75 | 0 | 0.25 | 0 |
| BH-TM1 | qPCR | 1.00 | 1.00 | 1.00 | 0.75 | 0.25 | 0 | 0 |
| BH-TM2 | qPCR | 1.00 | 1.00 | 1.00 | 0.75 | 0 | 0 | 0 |
| BH-TM4 | qPCR | 1.00 | 1.00 | 1.00 | 0.75 | 0 | 0.25 | 0 |
| Estimated Target-Sequence Copy Number | 2193 | 230 | 16 | 2.2 | 1.1 | U | U | |
| Bighead and Silver Carp: | ||||||||
| AC-6 | cPCR | 1.00 | 1.00 | 1.00 | 0.50 | 0 | 0.25 | 0 |
| AC-TM1 | qPCR | 1.00 | 1.00 | 1.00 | 0.50 | 0.50 | 0 | 0 |
| AC-TM2 | qPCR | 1.00 | 1.00 | 1.00 | 1.00 | 0.50 | 0 | 0 |
| AC-TM3 | qPCR | 1.00 | 1.00 | 1.00 | 0.75 | 0 | 0 | 0 |
| Estimated Target-Sequence Copy Number | 6051 | 508 | 46 | 3.4 | 1.6 | U | U | |
Sensitivity testing of each cPCR and qPCR marker across a dilution series of genomic DNA. The proportion of detections out of four replicates is noted for each marker at each dilution level. Estimated marker copy numbers per dilution are noted below each set of markers based on averages calculated across all replicates of qPCR sensitivity trials using a plasmid DNA standard. AC markers were tested using SC dilutions. U = Undeterminable copy number.
*Variation in amplifications at these levels are likely due to stochasticity of PCR at such low DNA concentrations.