Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jan 2;4(3):151–163. doi: 10.1016/j.molmet.2014.12.012

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Double knockdowns in myoblasts recapitulate the IR phenotypes predicted by in silico experiments. (A) Schematic of PDK2/4 regulation of PDH. (B) mRNA expression by qRT-PCR analysis. (C) Western blot analysis of phosphorylated and total PDH and ETFDH protein expression. (D) Changes in ATP levels (normalized to protein). (E) Changes in citrate synthase (CS) activity (normalized to protein). (F) Changes in ECAR/OCR values (normalized to DNA) under basal conditions (250 μM palmitate) and after addition of 5.5 mM glucose, shown relative to the basal level. *p < 0.05.