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. 2015 Jan 2;4(3):151–163. doi: 10.1016/j.molmet.2014.12.012

Table 1.

A. Major qualitative changes induced by increased glucose and fatty acid substrate availability (1xFA = 0.3838 mmol/L; 1xGluc = 5.0 mmol/L). B. Top KDs causing reduced metabolic flexibility in basal nutrient condition (1xFA-1xGluc). For metabolic flexibility, data indicate cumulative difference in RQ (Eq. 8 in Supplemental Experimental Procedures). For each KD, data indicate ratio of KD compared to control (Eq. 6 and 7 in Supplemental Experimental Procedures).

Condition Major qualitative changes compared to 1xFA-1xGluc
A.
2× glucose
  • Increased:
    • •
      Glycolytic fluxes
    • •
      Glycogen storage
    • •
      TAG storage
2× fatty acid
  • Increased:
    • •
      TCA
    • •
      OXPHOS
    • •
      Glycogen storage
    • •
      TAG storage
2× fatty acid-2× glucose
  • Increased:
    • •
      Glycolytic fluxes
    • •
      TCA
    • •
      OXPHOS
    • •
      Glycogen storage
    • •
      TAG storage
KD Reaction EC Number Met. Flex
 ATP + Cr-P Production
 Cumulative TCA Flux
Δ RQ Fasted Mixed Fed Fasted Mixed Fed
B.
PDH 1.2.4.1 0.09 0.94 0.78 0.80 0.93 0.77 0.73
FAT-CD36 n/a 0.03 0.75 0.92 1.00 0.74 0.91 1.00
PalTK 6.2.1.3 0.03 0.75 0.92 1.00 0.74 0.91 1.00
ASTM 2.6.1.1 0.01 0.96 0.73 0.68 0.97 0.91 0.64
ASTC n/a 0.01 0.95 0.73 0.68 0.96 0.72 0.64
Glu–Asp Ex n/a 0.01 0.96 0.73 0.68 0.97 0.72 0.64