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. 2015 Jan 16;4(3):210–221. doi: 10.1016/j.molmet.2015.01.004

Figure 4.

Figure 4

CNS NPY and Y1 receptor signaling promote expression of ARF-1 and lipin-1. The levels of mRNA and protein of ARF-1 and lipin-1, two key regulatory proteins involved in PL remodeling, were assessed from livers of 4-h fasted lean rats (n = 5–7/group) isolated 60 or 120 min after icv treatment with NPY (black bars), Y1 receptor agonist (gray bars) or Veh (white bars). Relative mRNA levels for liver ARF-1 (A) and lipin-1 (D) were obtained by quantitative RT-PCR. Individual mRNA levels for the protein of interest were first normalized to a non-regulated reference RNA, RPL13A, and then normalized to the Veh group for comparative analysis. Protein extracts prepared from livers of 4-h fasted lean rats isolated 120 min after icv injection of NPY or Y1 receptor agonist or Veh were immunoblotted to detect levels of ARF-1 (B and C) and lipin-1 (E and F). Images of Western blots [representative blots shown for ARF-1 (B) and Lipin-1 (E)] were analyzed by densitometry and data shown relative to Veh levels after normalization to loading controls, β-Tubulin or GAPDH. Data are presented as mean ± SEM and were analyzed by Student's t-test (unpaired, two-tailed); *indicates a significant difference (p < 0.05) between icv treatment and Veh.