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. 2015 Jan 16;4(3):210–221. doi: 10.1016/j.molmet.2015.01.004

Figure 6.

Figure 6

Hepatic sympathetic denervation (Sx) attenuates the stimulatory effect of icv NPY treatment on liver SCD-1 and lipin-1 expression. Lean 4-h fasted Sham or Sx rats received either a 2-h icv infusion of NPY (black bars; n = 5–6/group) or Veh (white bars; n = 6–7/group). Data are presented as mean ± SEM and were analyzed by one-way ANOVA with Bonferroni's post-test analysis; *p < 0.05. (A) Liver norepinephrine levels of all treatment groups are shown. (B) Relative gene expression in liver is shown for SCD-1 mRNA that was assessed by quantitative RT-PCR and normalized to the expression level of a reference gene, RPL13A. For comparative analysis, SCD-1 mRNA ratios were normalized to that of the Sham Veh group. (C) Protein extracts prepared from livers were immunoblotted and analyzed by densitometry to detect expression levels of lipin-1, which were normalized to a loading control, constitutive cytosolic Hsc70 (HSP73). For comparative analysis of lipin-1 expression, levels of each group were normalized to the Sham Veh group.