FIGURE 2.

Stability of circRNAs. (A) The RNA levels relative to day 1 of transfection were measured by qRT-PCR using primer sets for linear or circRNAs. The transfections were conducted with minigene containing structured intron, and all experiments are performed in triplicates with the means and SD of RNA levels plotted. In all panels, the RNA level of GAPDH was used to calibrate all other RNA levels. (B) Change of RNA levels after transcription inhibition by actinomycin D or DMSO control. Two minigenes containing structured or unstructured intron were transfected into 293T cells, and the levels of linear or circular RNAs were determined at different times after drug treatment. The level of a typical endogenous mRNA, SRSF1, was also measured as a control.