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. 2015 Feb;21(2):202–212. doi: 10.1261/rna.048199.114

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© 2015 Alings et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 7. — 2-Thiolation levels are not affected by temperature in most other yeast species. Representative autoradiogram images of tRNA extracted at different growth temperatures from (A) S. bayanus, (B) S. mikatae, (C) S. paradoxus, (D) C. glabrata, and (E) S. pombe following APM-affinity gel electrophoresis and Northern blot with the tK^UUU probe. 2-Thiolation levels were quantified by image densitometry (n = 3, apart from samples grown at 25°C where n = 2). Asterisks (*) show unspecific binding of the probe.