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. 2015 Feb;21(2):296–305. doi: 10.1261/rna.047159.114

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© 2015 Capece et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 4. — (A) Experimental setup for ZMW-based single-molecule SNAP_f experiments. (B) Full-chip average fluorescence values for SNAP_f translation detected by ZMW. When the reaction is treated with a translation inhibitor, GFP is substituted for SNAP_f, or plasmid is omitted, fluorescence decreases significantly. (C) The fluorescent signal from an individual ZMW well. Colocalization of red Cy5 signal (Cy5-Z50S) to green DY549 signal (SNAP_f) is observed, and the dyes photobleach after 200 and 100 sec, respectively.