Fig. 4.

JNK Pathway is sufficient for Scarface expression. (A–F) Whole mount third instar imaginal discs from third instar larvae capable of activating the JNK pathway in a Ptc-Gal4 driver pattern along the antero-posterior compartment border. The domain of Ptc-Gal4 expression is marked by RFP (red channel in A, D) from a UAS-RFP transgene. The Scarface expression is marked with GFP (green channel, arrows in B and E) from sf^pbss. The discs are counterstained with DAPI (blue) to label the nuclei. The merge of individual channels is also shown. Complete genotype of the discs can be found in materials and methods section. A–C are wing discs and D–F are leg and haltere discs labelled as L and H respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)