Fig. 4.
The interaction between the SH3 domain of Hck and the polyproline motif of ELMO1 is of moderate affinity. Titration of the 15N-labeled SH3 domain of Hck with increasing amounts of the ELMO707–727 peptide (up to 55 equivalents). The titration curves result from the fitting of the chemical shift variations of the aspartate 95, serine 110 and 129, tryptophan 113 and the glutamate 112 as a function of the ELMO707–727 peptide concentration. These variations were measured for the five different residues and the dissociation constant was calculated as described in the Section 2.