Figure 4.

Expression analysis of frog IL-10 in different tissues from healthy frog (a) and LPS stimulated frog (b). Six tissues including liver, spleen, kidney, intestine, heart, and stomach were sampled for real-time PCR analysis. The mean ± SD values were shown. The transcripts levels of IL-10 in healthy frog were relative to those of β-actin. The expression changes of IL-10 in LPS stimulated frog were expressed as fold change relative to the controls. P values generated by paired sample t-test between control and stimulated groups were shown above the bars. ^* P < 0.05.