A, Reaction scheme and proteins. B, D-loop dissolution by Sgs1, Sgs1-Top3-Rmi1 (STR), Sgs1-K706A-Top3-Rmi1 (DTR), Top3-Rmi1 (TR) (0, 2, 5, 10, 20, 50 nM). C, Quantitation of D-loops. Shown are normalized means ± standard deviations of three independent experiments. The absolute values corresponding to maximal D-loop levels are Sgs1: 14%, DTR: 12%, STR: 11%, and TR: 18%. The Sgs1 data were taken from Figure 1. D, D-loop dissolution by Top3 and Top3cd (Top3-Y356F) (0, 5, 10, 20, 50 nM). E, Quantitation of D-loops. Shown are normalized means ± standard deviations of three independent experiments. The absolute values corresponding to maximal D-loop levels are Top3: 22%, Top3cd: 19%.