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. Author manuscript; available in PMC: 2016 Feb 19.
Published in final edited form as: Mol Cell. 2015 Feb 19;57(4):595–606. doi: 10.1016/j.molcel.2015.01.022

Figure 2. Topoisomerase activity is necessary and sufficient for dissolution of Rad51–Rad54 reconstituted D-loops by Sgs1-Top3-Rmi1.

Figure 2

A, Reaction scheme and proteins. B, D-loop dissolution by Sgs1, Sgs1-Top3-Rmi1 (STR), Sgs1-K706A-Top3-Rmi1 (DTR), Top3-Rmi1 (TR) (0, 2, 5, 10, 20, 50 nM). C, Quantitation of D-loops. Shown are normalized means ± standard deviations of three independent experiments. The absolute values corresponding to maximal D-loop levels are Sgs1: 14%, DTR: 12%, STR: 11%, and TR: 18%. The Sgs1 data were taken from Figure 1. D, D-loop dissolution by Top3 and Top3cd (Top3-Y356F) (0, 5, 10, 20, 50 nM). E, Quantitation of D-loops. Shown are normalized means ± standard deviations of three independent experiments. The absolute values corresponding to maximal D-loop levels are Top3: 22%, Top3cd: 19%.