A, Reaction scheme for Rmi1-stimulated reactions. B, D-loop dissolution by Top3 or Top3-Rmi1 (0, 2, 5, 10, 20 nM). C, Quantitation of D-loops. Shown are normalized means ± standard deviations of three independent experiments. The absolute values corresponding to maximal D-loop levels are Top3: 21.1%, Top3-Rmi1: 18.7%. D, Reaction scheme with tailed 95-mer. E, D-loop dissolution time course by 2 nM Top3-Rmi1. F, Quantitation of D-loops. Shown are means ± standard deviations of three independent experiments.