Table 1. Glutamate sensitivity of mutant AVR-14B GluCls.
| Glutamate | Ivermectina | |||||
|---|---|---|---|---|---|---|
| EC50 (mM) | nH | Imax (μA) | n | Imax (μA) | n | |
| Wild-type | 0.04 ± 0.01 | 1.7 ± 0.2 | 3.1 ± 0.3 | 4 | 2.4 ± 0.4 | 5 |
| R95A | b | b | 0.04 ± 0.02c | 3 | 4.8 ± 1.9 | 3 |
| S169A | 27 ± 4*** | 1.8 ± 0.2 | 4.3 ± 1.1 | 7 | ||
| Y199F | b | b | 0.16 ± 0.04*** | 6 | 0.7 ± 0.2 | 2 |
| T245A | 16 ± 0.8*** | 2.0 ± 0.1 | 5.5 ± 0.8 | 7 | ||
| Y248F | 0.07 ± 0.01 | 1.9 ± 0.2 | 4.3 ± 0.8 | 6 | ||
| R76N | b | b | 0.19 ± 0.08c | 6 | 3.6 ± 1.0 | 4 |
| Q141R | 6 ± 1** | 0.9 ± 0.1** | 1.1 ± 0.4** | 5 | ||
| K219A | 0.03 ± 0.01 | 2.1 ± 0.4 | 3.3 ± 0.5 | 3 | ||
| RN/QR/KA | 34 ± 3*** | 0.9 ± 0.1** | 2.3 ± 0.3 | 5 | ||
| A197E | b | b | 0.01 ± 0.01c | 6 | 2.4 ± 0.7 | 4 |
Mean ± s.e.m. from n experiments.
a1 μM ivermectin was applied to oocytes at which glutamate gated little or no currents (and to WT-expressing oocytes), in order to verify functional expression of GluCls.
bCould not be determined.
cNot significantly different from zero (one-sample t test).
**P < 0.01,
***P < 0.001, compared to WT (unpaired Student's t test).