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. 2014 Nov 11;1(1):55–74.e1. doi: 10.1016/j.jcmgh.2014.08.001

Figure 8.

Figure 8

Lentiviral cathelicidin expression reduces colonic inflammation in Salmonella-induced cecal inflammation in mice. (A) Illustration of the experimental plan of Salmonella-mediated cecal inflammation with LV vector protocol. Mice were fed with streptomycin to reduce the microflora load in the intestine and facilitate infection, and 24 hours later, the mice were inoculated with Salmonella. Some mice received intravenous injection of mouse cathelicidin Camp-HA-expressing lentivirus or control lentivirus on day 11. Cecal changes were evaluated on day 21. (B) Cecal Camp mRNA expression was statistically significantly increased in the Salmonella-infected group and was further augmented in the Camp-LV group (P = .0001 and P = .0317). (C) Representative H&E images of colonic tissues. (D) Histology score was based on H&E staining images of colonic tissues. TNBS treatment in mice led to increased tissue damage with a statistically significantly higher histology score (P = .0001) compared with the ethanol control. Treatment of Camp-LV statistically significantly reduced histology score (P = .0001). (E) Salmonella infection statistically significantly induced cecal Tnfα mRNA (P = .0001) expression. Camp-lentivirus treatment significantly reduced Salmonella induced Tnfα mRNA (P = .0107) expression. Per group, n = 6.