Table 1. Data collection and refinement statistics.
| Statistics | Aquifex aeolicus MinD | Aquifex aeolicus MinCD complex |
|---|---|---|
| Protein | MinD( Δ C13)-GSHHHHHH | MinD(Δ C12), D40A MinC: 82-201-GSHHHHHH |
| UniProt IDs | O67033_AQUAE | O67034_AQUAE, 067033_AQUAE |
| Data collection | ||
| Beamline | ESRF ID23eh2 | ESRF ID23eh1 |
| Wavelengths (Å) | 0.8726 | 0.9795 |
| Crystal | ||
| Space group | P1 | P6522 |
| Cell (Å) | 36, 52, 69; 71°, 89°, 70° | 130.6, 130.6, 298.5 |
| Scaling | ||
| Resolution (Å) | 1.9 | 2.7 |
| Completeness (%)1 | 93.1 (92.3) | 100.0 (100.0) |
| Multiplicity1 | 2.4 (2.4) | 14.3 (12.9) |
| (I) / σ(I)1 | 8.8 (2.6) | 20.6 (4.6) |
| Rmerge1 | 0.120 (0.470) | 0.096 (0.629) |
| Rpim1 | 0.097 (0.379) | 0.026 (0.179) |
| Refinement | ||
| R / Rfree2 | 0.216 (0.279) | 0.1907 (0.2357) |
| Model | 2 × MinD 2-40, 44-87, 96-248, 2 ADP, 2 Mg, 135 H2O | 2 × MinC, 2 × MinD, D: 2-88, 95-251, 2 ATP, 2 Mg; C: 84-205 |
| Bond length rmsd (Å) | 0.014 | 0.016 |
| Bond angle rmsd (°) | 1.591 | 1.630 |
| Most favoured (%)3 | 92.4 | 88.6 |
| Disallowed (%)3 | 0 | 0 |
| PDB ID | ||
| 4V03 | 4V02 |
1
Values in parentheses refer to the highest recorded resolution shell.
2
5 % of reflections were randomly selected before refinement.
3
Percentage of residues in the Ramachandran plot (PROCHECK).