Skip to main content
. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Nat Immunol. 2014 Oct 19;15(12):1116–1125. doi: 10.1038/ni.3023

Figure 3. Ym1 promotes OVA-induced neutrophilia and regulates IL-17A abundance.

Figure 3

(a-c) Cells from BAL of PBS or OVA-challenged mice treated with IgG2a or anti-Ym1 (200 μg, i.p.). showing total numbers and percentage of eosinophils and neutrophils as calculated from DiffQuick stained cytospins. (b) IL-17A secreted from thoracic lymph node cells cultured with OVA antigen (Ag, 500 μg) or α-CD3 (1 μg/mL) from mice as in a. Data show IL-17A responses normalized to amount of IL-17A secreted in medium controls for each individual mouse. (c) Gene expression in lung tissue of mice as in a. ND, not detected. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 (analysis of variance with (a, d) Tukey-Kramer HSD multiple comparison test or (c) Kruskal-Wallis test). Data are pooled (a; mean ± s.e.m.) or representative (c, d; mean ± s.e.m.) from two independent experiments of eleven to twelve mice per group.