Figure 5. Bcl11a is required in DMBA-mediated tumourigenesis.

(a) Mouse images and fat pads whole-mount fat pads of either WT, flox/flox, p53flox/flox or Dflox/flox mammary cells. (scale bar, 500 μm) (b) Quantification of the tumours with DMBA-mediated tumour development from the four depicted groups of engrafted cells over a period of 26 weeks. A log-rank (Mantel–Cox) test was used to compare the two groups and calculate the P value. (c) Tumour size quantification of DMBA-mediated tumours in WT, +/flox and flox/flox mice. Mice were checked regularly for tumour development and once detected, Cre activation was induced using three injections of tamoxifen (first day of injection is indicated by black arrow). Tumours size was then monitored for up to 20 days or until they reach a critical size.