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. 2015 Jan 9;6:5987. doi: 10.1038/ncomms6987

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(a,b) X-gal staining of a 12.5dpc embryo and whole mount of the mammary gland from a 5-week-old Bcl11a-^lacZ/+ female virgin mouse. The dashed circles highlight the mammary placodes and the terminal end buds. LN: lymph node. (scale bar, 500 μm) (c) qRT–PCR for Bcl11a in different mammary epithelial cell compartments that were fluorescence-activated cell sorting-purified using antibodies for CD24, CD49f and CD49b. Data are presented as mean±s.d. (n=3) and t-test was performed and the P values are displayed on the plot. (d) Depletion of the MaSCs-enriched population (CD24^medCD49f^hi, dashed lines) in the Bcl11a-deficient mammary gland detected by flow cytometric analysis. (e) Limiting dilution transplant (fat pad) assay showing severely compromized engraftment of Bcl11a-deficient MaSCs. Stem cell frequency calculation is described in the Methods.