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A positively charged lysine residue placed N-terminal to a 4D stretch reduces the electric pulling force. fFL values are shown for 4D-SecM(Ms) constructs with Asn, Lys, His, and Pro residues included in the indicated positions. In all cases, the number of residues between the indicated stretch of residues and the P-site is L = 43 residues. Standard errors (s.e.m.) are indicated.
