Table 3.
Glycan type abundance in hFSH oligosaccharide preparations.
| Glycan Type | Relative Abundance (%) | |||
|---|---|---|---|---|
| Pituitary hFSH | Pituitary hFSH [54] | Pituitary hFSH [55,56] | Urinary hFSH | |
| High-mannose | 0 | 0 | 0 | 0 |
| Biantennary | 38.2 | 46 | 36 | 37.2 |
| Triantennary (3)a | 41 | 30.3 | 49 | 44 |
| Triantennary (6)b | 0 | 17.2 | 0 | 0 |
| Tetra-antennary | 15 | 5 | 0 | 14.8 |
| Complex | 94.2 | 98.5 | 85 | 95.9 |
| Neutral | 0.3 | 5 | 10 | 2.2 |
| Sialylated | 99.1 | 95 | 88 | 97.5 |
| Sulfated | 6.5 | 0 | 7 | 4.2 |
| Sial/Sulfate | 5.9 | 0 | 5 | 3.9 |
| Fucose | 43 | 51 | NDc | 23.9 |
| Antenna-Fucose | 0.3 | 0 | 0 | 0 |
| Bisecting | 32.6 | 21 | 34 | 23.9 |
| GalNAc | 2.8 | 0 | 7 | 1.7 |
| FSH Preparation | AFP7298A | Renwick | NIAMDD-hFSH-2 & AFP4822B | ProSpec |
| FSH Potency | 8560 IU/mg | >1950 IU/mg | 3100 & 3925 IU/mg | 10,000 IU/mg |
a
Triantennary (3); third glycan branch on Man(α1-3)Man
b
Triantennary (6); third glycan branch on Man(α1-6)Man
c
ND; not determined