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. 2014 Oct 1;64(3):897–903. doi: 10.2337/db14-0684

Figure 1.

Figure 1

Nkx6.1 deletion in newly formed β-cells leads to glucose intolerance and reduced β-cell mass. A: Schematic of alleles and transgenes used to inactivate Nkx6.1 in fetal β-cells. Rectangles show coding sequences; triangles show loxP sites; red rectangle shows DsRed coding sequence. B and C: Immunofluorescence staining for Nkx6.1 and insulin reveals loss of Nkx6.1 in most β-cells of Nkx6.1∆β mice at P0. D: Blood glucose levels in 6-week-old Nkx6.1∆β mice fed ad libitum compared with control mice (n = 6). E: Intraperitoneal glucose tolerance test shows glucose intolerance in 6-week-old Nkx6.1∆β mice as compared with control mice (n = 6). F: Quantification of β-cell mass reveals decreased β-cell mass in Nkx6.1∆β mice at 6 weeks of age (n = 3). Data shown as mean ± SEM. Scale bars = 20 μm. Ins, insulin; YFP, yellow fluorescent protein. *P < 0.05; **P < 0.01.