Extended Data Figure 3. Cooling does not induce changes in PrP^Sc or Aβ levels.

a) Levels of total PrP (upper blot) and PrP^Sc (lower blot) do not change notably before (white line), during (blue line) or after (red line) cooling to 16-18°C in prion-infected mice. PrP^Sc is detected after digestion with proteinase K. Levels are undetectable by western bloting at 6 w.p.i., as expected. b) Cooling does not change levels of Aβ oligomers in 5×FAD mice, arrow indicates Aβ monomers (Lane 1 - synthetic Aβ oligomers, last lane - one year old 5×FAD control (C+)). Representative western blots are shown for 3 mice per temperature and time point. Data in bar charts are mean ± s.e.m. Student’s t-test, two tailed. Non-significant p values.