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. Author manuscript; available in PMC: 2015 Feb 24.
Published in final edited form as: Blood. 2012 Sep 12;120(18):3803–3811. doi: 10.1182/blood-2012-03-419663

Fig. 2. CA-WASp requires Arp2/3 activity to increase F-actin production.

Fig. 2

(A) Percentage increase in total cellular F-actin due to CA-WASp, measured by flow cytometry in HT1080 cells (blue) and U937 cells (red) cultured with DMSO, 20 μM or 40 μM CK666. n = 3 mean +/− SD. (B,C) Confocal images of (B) interphase HT1080 and (C) prometaphase U937 cells showing DNA (DAP1, blue), GFP-CA-WASp (green), and F-actin (red). Dashed white circles show individual ~ 10 μm2 areas used to measure F-actin density. Bar = 10 μm. (D, E) Nuclear F-actin density in prometaphase (D) HT1080 and (E) U937 cells with and without CA-WASp expression cultured in the conditions shown, n > 10 for each condition. Confocal microscopy was performed with a Zeiss LSM 710 inverted confocal microscope with a 40x C-Apochromat NA 1.2 WD 280 mm objective. Image analysis used imageJ software. Fluorochromes were DAP1, eGFP-WASp and Alexafluor-647-phalloidin.