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. Author manuscript; available in PMC: 2015 Feb 24.
Published in final edited form as: Blood. 2012 Sep 12;120(18):3803–3811. doi: 10.1182/blood-2012-03-419663

Fig. 3. Arp2/3 complex inhibition rescues the proliferative and nuclear defects caused by CA-WASp.

Fig. 3

(A, B) Percentage of (A) U937 and (B) HT1080 cells expressing GFP-CA-WASp 3, 6, 8, 10,13 and 15 days after transduction cultured with DMSO, 20 μM or 40 μM CK666, n = 3 mean +/− SD. (C) Apoptosis measured by flow cytometry of annexinV stained U937 cells after culture for 3-8 days in the conditions shown, with (black) and without (grey) CA-WASp expression, n = 8. Percentage of binucleated (D) U937 and (E) HT1080 cells after culture for 3-10 days in the conditions shown, n = 8. Control (grey bars), CA-WASp (black bars). (F, G) Percentage of micronucleated (F) U937 and (G) HT1080 cells after culture for 3-10 days in the conditions shown, n = 6. Black represents cells with micronuclei alone, grey represents binucleated cells with micronuclei. (H) Lagging chromosomes at anaphase in HT1080 cells. Bar = 10 μm. (I) Percent of HT1080 cells with lagging anaphase chromosomes n = 3 with at least 300 cells analyzed per condition. Confocal microscopy was performed as in Figure 2.