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. 2015 Feb 13;14:19. doi: 10.1186/s12934-015-0202-z

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© Della Cristina et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Cleavage pattern assessment of secreted PE. (A) Western blot analysis of native PE fragments derived from PE cleaved under different conditions. CTR (control): native PE incubated with PBS; αA BMMY: native PE incubated with BMMY after 48 h of induction of the GS115 mock transformant pPICZαA (αA) clone; αA BMMY PMSF: as αA BMMY but PE was incubated in addition + 1 mM PMSF BMMY: induction medium only. (B) In silico study of identifiable furin-like cleavage sites in the native PE sequence.