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4KB-SAP and 4KB-PE40 fusions expressed in Pichia pastoris G115 ( his4 ). (A) Schematic representation of saporin- (C1-9) or PE-based (10) -4KB128-derived fusion constructs. C1: Pichia-fully optimized Construct 1 expressing clone. (B) Western blot analysis of 4KBopt218L-SAP clones. Secretion yields were estimated at 1-2 mg/L and compared to induced mock or positive control anti-PA63scFv-SAP expresser clones.
