Figure 3.

Kras; p16p19^null tumor cells repopulate tumors in secondary recipients more effectively than SmoM2 mouse tumor cells. Pi⁻Ca⁺GFP⁺ Kras; p16p19^null tumor cells were sorted independently from two primary tumors and injected into the cardiotoxin-pre-injured gastrocnemius muscles of NOD.SCID mice (50 cells per injection). Pi-Ca+ SmoM2 tumor cells were sorted independently from four primary tumors and injected into the cardiotoxin-pre-injured gastrocnemius muscles of NOD.SCID mice (100,000–150,000 cells per injection). Recipient mice were monitored for the occurrence of secondary tumors at the injection site for up to 4 months.